V.P. Stus, I.S. Shponka, K.S. Barannik, O.V. Poslavska

Violation of the local kidney blood-groove when modelling unilateral pathological processes in kidneys

In experiment on 24 white polovozrelykh rats of the line Vistar created constant ischemia of the left kidney by a perevyazyvaniye of her artery on 15 rats (group A), sharp violation of an urodinamika of the left kidney by a perevyazyvaniya of a mochetochnik at the level in / 3 on 4 rats (group B) and modelled both pathological states on 5 rats (group C). The condition of a parenchyma of a kidney of ten rather healthy rats was taken for control (control group) for comparison. At histologic research the main monoclonal antibodies to CD3 (an inflammatory marker) and aSMA (a vascular marker were used) (TermoScientific, the USA).

In experimental kidneys of subgroup of A I (the left kidney) which was under the influence of experimental ischemia, at histologic research a large number full-blooded the dilyatirovannykh of vessels is revealed, revascular hypostasis of a stroma and izvity the course was noted. An epithelium of the majority of tubules were in a condition of gidropichesky dystrophy. The maintenance of CD3+ of T-cages for subgroup A I made 62,7±1,5. In subgroup B I (urostaz the left experimental kidney) on histologic cuts the atrophy of tubules among sclerous an interstition, the centers of a chronic inflammation with the most part of CD3+ of T-lymphocytes was observed. The quantity of CD3+ of T-cages for subgroup B I made 54,1±0,9. In experimental kidneys of subgroup of C I the heaviest are revealed pathological violation in a parenchyma which were shown gidronefrozy, by a sclerosis, an inflammation with defeat of vessels (a dilyatation, hemorrhage, interstitsialny I swelled). The maintenance of CD3+ With I made T-lymphocytes for subgroup 58,9±01,1.

The main pathological changes in contralateral “healthy” kidneys of the studied groups of rats were shown available dystrophic changes in an epithelium of tubules, the centers of hypostasis and a sclerosis round large vessels, the peretubulyarnykh and revascular the kruglokletochnykh of inflammatory infiltrates with the maintenance of CD3+ of T-cages (for subgroup A II - 10,3±1,2; for В II - 11,9±1,1; for С II - 10,9±0,8) which differed from control group (р < 0,05) where CD3+ T-lymphocytes met in the form of single cages an interstition at the level of 2,1±0,5.

Thus, unilateral damage of kidneys at rats, owing to experimental ischemia or violation of an urodinamika or at the same time two pathological states leads to sharp violation of a local kidney blood-groove. In contralateral “healthy” kidneys also there are pathological changes even before carrying out a nefrektomiya that indicates the need of their medicamentous support.