V.P. Stus, I.S. Shponka, V.O. Bondarєva, V.M. Krasnov

Morfological and morphometric changes in the kidneys of pregnant female rats under the influence of heavy metal salts and possible protective effect nanometals

The study of the effects of heavy metals on organs and tissues condition of humans and animals is a leading question. The main danger of heavy metals is that they are capable of gradually accumulate in the food chain and, thus, a greater or lesser degree of influence on the human body. Getting in person’s organіsm, even in small doses, the compounds accumulate in it and almost alone is not displayed. Among these compounds one of the foremost is the lead and its modified forms.

Despite the considerable progress that has been made in identifying various extracellular and intracellular membrane ligands, which play an important role in the development of mechanisms of nephrotoxicity, many questions etiopathogenetic kidney damage stay quite controversial and require further research.

For experimental studies were selected female coloring. After the adaptation period, which was 12 days, animals with a persistent rhythm of the estrous cycle at the age of 3-3.5 months., Weighing 170-200 g of 70 in a stage of proestrus and estrus s intact males were mated according to the scheme 2:1. The first day of gestation were determined by the presence of sperm in vaginal smears. Female rats with dated term pregnancy distributed on 7 groups, of which 6 - experienced (8 females in each group) were administered drugs in isolation (group 1 - lead acetate at a dose of 0.05 mg/kg, group 2 - zinc chloride at a dose of 1.5 mg/kg, group 3 - zinc citrate, obtained by nanotechnology in a dose of 1.5 mg/kg, 6 group - lead citrate, resulting Square nanotechnology in a dose of 0.05 mg/kg) in combination (group 4 - lead acetate and zinc chloride, 5-group - lead acetate that nanozinc) and one (7th group) - a control that received demineralized water.

It is established that under the action of the toxic heavy metal compounds renal morphological changes were observed in all structures of the body not only in the epithelium of the proximal and distal tubules, but also in the renal glomerulas. And the most striking effect on the epithelium of the renal tubules of the experimental animals was observed in the 4th group with a combination of the effect of salts of heavy metals, namely lead acetate and zinc chloride. The best results in all parameters were obtained in groups of animals that have lain down action of toxic compounds using nanometals (3rd and 6th group), indicating that the protective effect nanotehnology by stabilizing membranes.