F.I. Kostev, R.V. Bakhchiev, K.V. Litovkin

Molecular and genetic monitoring of early diagnosis of prostate cancer

This paper presents the results of studying the level of methylation of GSTP1 gene promoter region in patients with histologically verified prostate cancer, BPH and BPH combination with such transition state as PIN and ASAP. There were examined 80 patients, including 45 with BPH and 35 with prostate cancer. The object of the study was the tissue obtained by means of performing multifocal transrectal prostate biopsy. It was found out that methylation of GSTP1 gene according to our data, is intrinsic to 85.7% of prostate adenocarcinomas. The calculated sensitivity and specificity was based on the method for the diagnosis of prostate cancer gene GSTP1 methylation with the resulted level of 60% and 94% respectively. It was found that the average level of methylation of GSTP1 gene in prostate cancer was significantly higher than in BPH. It was also found that the average level of methylation in BPH combined with PIN or ASAP was different, being higher than the typical BPH, but lower than in prostate cancer. Defined thresholds levels of methylation of the gene GSTP1 promoter region in various pathological conditions that can be used as criteria for differential diagnosis of precancerous conditions and early detection of prostate cancer.